Genital functional morphology, hypothesized plugging methods, and mating positions

On the latter instance, we therefore could separate both main spots and you can ?step one plot on each section of the membranous cells ( Profile step 1 and you will Dining table step one)

From inside the Meters. seminigra queens, i noticed each other single few and you can several pairs from melanized spots ( Profile step one and you will Supplementary Shape S4 ), and we never ever located long lasting mating plugs within this kinds. The newest shipment out-of several patches ranged between step three and you will seven individualized portion, with different kind of preparations. In the 33 M. seminigra specimens assessed, fifteen demonstrated unmarried matched up patches pattern, several demonstrated just one matched patches in conjunction with a main plot, and you may six specimens showed a simultaneous patch development ( Secondary Contour S4 ). On these agreements, the main melanized patches matched the latest inferred position of the spatha-brand new central build in the mating connect ( Profile 2E,I). It’s value discussing that individuals caught an additional recently mated Meters. seminigra king, that was holding 2 mating plugs: step 1 is actually connected in her genital chamber, although the almost every other try on the additional epidermis away from this lady gut, between sterna IV and you may V ( Supplementary Shape S4A ). That it recently mated was not fully physogastric, which means wasn’t utilized in the main dataset.

Male genital product and you can vaginal sternum away from M. fasciculata. Ventral view of genital pill, metasomal sternum S-VIII, and metasomal sternum S-IX, given that found into the virgin males (A). Ventral look at metasomal sternum S-VIII (B) and you will metasomal sternum S-IX (C). Genital capsule into the ventral check, exhibiting new gonocoxites and a set of gonostyli (D). Vaginal capsule in the dorsal look at, presenting the fresh spatha and you may a pair of dick regulators (E). Genital product from inside the ventral take a look at, proving during the series, the latest rotation of the dick valves, in advance of gonocoxites lateral compressing (F–H). Vaginal pill with caused dick regulators and you will gonocoxites compressed laterally, for the dorsal (I) and you can ventral look at (J).

Male vaginal pill and vaginal sternum of M. fasciculata. Ventral look at vaginal medicine, metasomal sternum S-VIII, and you may metasomal sternum S-IX, because the discover to the virgin boys (A). Ventral view of metasomal sternum S-VIII (B) and you can metasomal sternum S-IX (C). Vaginal capsule inside ventral glance at, indicating this new gonocoxites and a couple of gonostyli (D). Vaginal product in the dorsal look at, to present brand new spatha and you will a pair of penis valves (E). Genital tablet into the ventral take a look at, exhibiting for the sequence, the brand new rotation of the dick valves, prior to gonocoxites lateral compression (F–H). Vaginal supplement which have caused knob valves and you may gonocoxites compacted sideways, when you look at the dorsal (I) and ventral check (J).

Useful morphology of the male vaginal capsule

The male genitalia from Meters. fasciculata provides 3 components: 2 overlapping metasomal sterna (S-VIII and you will S-IX) and a genital supplement ( Profile dos). The fresh new vaginal tablet have a couple of nearly triangular gonocoxites, in which a pair of enough time gonostyli together with pair of piercing penis regulators are articulated; dorsally there is a narrow gonobase and you can an average spatha ( Profile 2E,I). These sclerites is actually highly kept together with her because of the a series of membranes that enable its 100 % free articulation. All the portion is firmly sclerotized, except for the fresh new putatively painful and sensitive setae into distal third of gonostyli; and are positioned ventrally in the male human anatomy.

The feminine genitalia regarding M. fasciculata is found in to the a great chamber formed of the tergum and you can sternum of your sixth intestinal segment ( Figure step three). The last portion of the ladies body is membranous, improperly sclerotized, kept of the pairs from horizontal hemitergites (T-VIII and you can T-IX). Amazingly arising from brand new membranous rear end, we come across a set of small setose gonostyli, a central bulb (the newest gonopore city), and you will a set of thin, lateral rami. Anywhere between such formations additionally the sternum S-VI underneath, following next the new gonopore town, you can find large membranous pouches-structure purse with just minimal sclerotization-to that your male mating connect try affixed during the mating ( Contour 3A,J) and copulatory scratching are found ( Supplementary Figure S5 ). Membranous pockets is illustrated by the a light place below the gonopore into the Contour 3. These types of membranous formations get inside Contour step one for everybody species, both in virgin and mated queens, even in the event their relative proportions and you may positioning can be seen in detail inside Secondary Contour S5 .